Our paper have been published in Optics Letters.

This research was based on the collaboration with Dr. Hirori (Kyoto U), Dr. Sakaguchi (Kyoto U) and Dr. Tachizaki (Tokai U) to elucidate the effects of THz pulse on gene networks in human induced pluripotent stem cells.

This paper has been introduced on iCeMS, Kyoto U website.

“Terahertz zaps alter gene activity in stem cells”

Title: Terahertz pulse-altered gene networks in human induced pluripotent stem cells

Authors: Takehiro Tachizaki, Reiko Sakaguchi, Shiho Terada, Ken-ichiro Kamei, and Hideki Hirori

Abstract: Terahertz (THz) irradiation has been exploited for biomedical applications in the non-invasive manipulation of living cells. Here, we develop an apparatus for studying the effects of THz pulse irradiation on living human induced pluripotent stem cells (hiPSCs). The spot of focused THz pulse covers the entire cell-culture area, where the maximum electric field reaching 0.5 MV/cm. RNA sequencing of global gene-expression analyses reveals that many THz-regulated genes are driven by zinc-finger (ZNF) transcription factors. These results suggest that the local intracellular concentration of metal ions, such as Zn2+, was changed by the effective electrical force of our THz pulse owing to its quasi-unipolar temporal shape.

DOI: 10.1364/OL.402815

Illustration © Mindy Takamiya

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