Cellular microenvironments play a crucial role in determining stem cell fates, yet conventional techniques offer limited control over these niches. Thus, there is a need for tools that can accurately regulate stem cells.

Microfluidic technologies excel in investigating niche roles on cells and tissues, as well as establishing in vitro cell-based assays, due to their precise control of liquid flow, 3D geometry creation, minimal reagent consumption, and automation capabilities. Notably, microfluidic devices enable the manipulation of both chemical and physical cellular conditions within a compact space, allowing for robust and high-throughput cell culture and assays. This makes microfluidic technology an ideal platform for recreating in vivo niche conditions and investigating unresolved niche mechanisms.

In previous research, I developed high-throughput microfluidic platforms to create multiple 3D artificial cellular environments, identifying optimal conditions for specific cellular functions. By utilizing this platform, we aim to screen artificial microenvironments that facilitate the differentiation of targeted tissue cells derived from human pluripotent stem cells (hPSCs).

Our findings will help establish new methodologies for precisely inducing hPSC differentiation and contribute to future hPSC applications in tissue engineering and regenerative medicine.

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