Our collaborative research (Dr. Hirai, Dr. Yang, Dr. Iida, and Prof. Tabata) got published in Communications Biology! Organs-on-a-Chip for in vitro model of non-alcoholic fatty liver disease/

Title: Integrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease

Authors: Jiandong YangYoshikazu HiraiKei IidaShinji ItoMarika TrummShiho TeradaRisako SakaiToshiyuki TsuchiyaOsamu Tabata & Ken-ichiro Kamei

Abstract: Non-alcoholic fatty liver disease (NAFLD) afflicts a significant percentage of the population; however, no effective treatments have yet been established because of the unsuitability of in vitro assays and animal experimental models. Here, we present an integrated-gut-liver-on-a-chip (iGLC) platform as an in vitro human model of the gut-liver axis (GLA) by co-culturing human gut and liver cell lines interconnected via microfluidics in a closed circulation loop, for the initiation and progression of NAFLD by treatment with free fatty acids (FFAs) for 1 and 7 days, respectively. Co-cultured Caco-2 gut-mimicking cells and HepG2 hepatocyte-like cells demonstrate the protective effects from apoptosis against FFAs treatment, whereas mono-cultured cells exhibit induced apoptosis. Phenotype and gene expression analyses reveal that the FFAs-treated gut and liver cells accumulated intracellular lipid droplets and show an increase in gene expression associated with a cellular response to copper ions and endoplasmic reticulum stress. As an in vitro human GLA model, the iGLC platform may serve as an alternative to animal experiments for investigating the mechanisms of NAFLD.

Communications Biology volume 6, Article number: 310 (2023)

Copyright of eye-catch image is belonged to Ms. Mindy Takamiya (CC BY-ND 4.0).


Principle investigator Associate Professor

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