Samah Abou-Sharieha

Postdoctoral Fellow


Education and Research Experience

Postdoctoral training, The Weizmann Institute of Science-Israel Feb, 2015-Mar, 2016

Research: circERBB2 role in breast cancer

Techniques: RNA harvest, circRNA expression profiling, exosomal isolation and

characterization, siRNA design for circRNA and proliferation assay.


Research-covering period, The Hebrew University of Jerusalem-Israel, Jan, 2014-Sep, 2014

Research: Impact of miRs in osteosarcoma on metastasis

Techniques: Lentiviral infection, adenoviral amplification, XTT assay, matrigel

invasion assay and intra-tibial injection


Postdoctoral training, The Ohio State University Medical Center (OSUMC)-USA, Aug, 2012-Aug, 2013

Research: Identification of lncRNA that has a role in glioblastomagenesis

Techniques: Sphere culture, RT-qPCR, immunohistochemistry, immunocytochmistry,

ELISA, IP/western blotting, FACS, in situ PLA, RIP and luciferase-reporter assay


PhD in Biotechnology, Okayama University-Japan Apr, 2006-Mar, 2009

Thesis title: Identification of cell surface marker candidates on breast cancer cells

using our originally fabricated microarray coupled with SOM analysis

Techniques: cell culture, microarray spotting and hybridization, DNA and RNA

isolation, self-organizing map (SOM) and ImageJ analysis, PCR, Real-time PCR,

fluorescence microscopy, western blotting, siRNA transfection and MTT assay


Publications

1) Abou-Sharieha S, Sugii Y, Touya, Dongwei Y, Ling C, Tokutaka H and Seno M.

Identification of TM9SF2 as a candidate cell surface marker common to breast carcinoma cells. Clin Oncol and Cancer Res. 2009; 6: 1-9

2) Seno M, Sugii Y, Touya, Abou-Sharieha S and Tokutaka H. Identification of tumor cell surface marker using sSOM analysis, chapter 7: Self-organizing maps, Springer,

Japan 2008

3) Salah Z, Arafeh R, Maximov V, Galasso M, Khawaled S, Abou-Sharieha S, Volinia S, Jones KB, Crove CM and Aqeilan RI. MiR27a and miR27a* contribute to metastatic properties of osteosarcoma cells. Oncotarget. 2015; 10; 6(7):4920-35


Posters

1) Abou-Sharieha S, Sugii Y, Tada H and Seno M. Identification of cell surface marker candidates in breast cancer using an oligonucleotide-based microarray

system. 66th Ann Meeting Jap Cancer Assoc proceedings. October 3-5, 2007, Yokohama, Japan

2) Abou-Sharieha S, Sugii Y, Tada H and Seno M. Two carrier genes; RFC1 and TM9SF2, are novel cell surface marker candidates in breast cancer. Amr Assoc

Cancer Res annual meeting (2008) proceedings. April 12-16, 2008, San Diego, California, USA

3) Abou-Sharieha S, Sugii Y, Tada H and Seno M. TM9SF2, a novel cell surface potential target in breast cancer. Second JCA-AACR Special Joint Conferences; The Latest Advances in Breast Cancer Research. July 14-16, 2008, Hyogo, Japan

4) Abou-Sharieha S, Sugii Y, Tada H and Seno M. TM9SF2, a novel cell surface potential target in breast cancer. 2008 NCRI Cancer Conference. October 5-8, 2008, Birmingham, UK

5) Abou-Sharieha S, Sugii Y, Tada H and Seno M. TM9SF2, a novel cell surface potential target in breast cancer. 20th EORTC-NCI-AACR symposium on

“Molecular Targets and Cancer therapeutics”. October 21-24, 2008, Geneva, Switzerland

6) Abou-Sharieha S, Sugii Y, Tokutaka H and Seno M. TM9SF2 Is Overexpressed in Breast Cancer and Is a Potential Cell Surface Target for Breast Cancer Gene

Therapy. Joint Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical Society, 2008. December 9-12, 2008, Kobe, Japan


Employment

1) Sep, 2018-now, Postdoctoral Fellow, Institute for Integrated Cell-Material Sciences, Kyoto University, Japan

2) Jul, 2016-now, Assistant Professor, The Faculty of Pharmacy, Amman University, Amman, Jordan

3) Nov, 2009-Jul, 2012, Lecturer, The Department of Clinical-Laboratory Sciences, Al-Ghad College of Medical Sciences, Jeddah, Saudi Arabia

3) Apr, 2006-Mar, 2008, Teaching Assistant, The Department of Biomedical Engineering, Okayama University

4) Apr, 2005-Mar, 2006, Teaching Assistant, The Department of Biochemistry, Okayama University


Awards and Internships

1) Japanese Ministry of Education Scholarship (Monbukagakusho) 2003-2009

2) Research intern in Katayama Chemical Industries CO., LTD, Japan 11th-22nd Dec, 2006

3) Visiting student at Prof Werner Schelgel lab, Geneva University, Switzerland 10th-20th Oct, 2008

4) Visiting researcher at Prof Samatey lab, Okinawa Institute of Science and Technology, Japan 18th Feb-5th Mar, 2009