Title: Terahertz pulse-altered gene networks in human induced pluripotent stem cells
Authors: Takehiro Tachizaki, Reiko Sakaguchi, Shiho Terada, Ken-ichiro Kamei, and Hideki Hirori
Abstract: Terahertz (THz) irradiation has been exploited for biomedical applications in the non-invasive manipulation of living cells. Here, we develop an apparatus for studying the effects of THz pulse irradiation on living human induced pluripotent stem cells (hiPSCs). The spot of focused THz pulse covers the entire cell-culture area, where the maximum electric field reaching 0.5 MV/cm. RNA sequencing of global gene-expression analyses reveals that many THz-regulated genes are driven by zinc-finger (ZNF) transcription factors. These results suggest that the local intracellular concentration of metal ions, such as Zn2+, was changed by the effective electrical force of our THz pulse owing to its quasi-unipolar temporal shape.
Image credit: Mindy Takamiya @ iCeMS, Kyoto University