九州大学前多研との共同研究論文が、Phys. Rev. Res.誌に受理されました！

九州大学前多准教授と白木くんとの共同研究が、Physical Review Research誌にアクセプトされました！

オンラインにすぐ掲載されますが、arXivからもご覧になれます。

Title: Randomness and optimality in enhanced DNA ligation with crowding effects

Authors: Takaharu Y. Shiraki,  Ken-ichiro Kamei, and Yusuke T. Maeda

Abstract:

Enzymatic ligation is essential for the synthesis of long DNA. However, a number of ligated products exponentially decay as DNA synthesis proceeds randomly. The control of ligation randomness is important to suppress exponential decay and achieve efficient synthesis of long DNA. This study analyzes the randomness in sequential DNA ligations, termed qPCR-based STatistical Analysis of Randomness (qPCR-bSTAR), using a probability distribution of ligated DNA length. Exponential decay is suppressed in a polymer solution. Further, DNA ligation is found to be activated at optimal crowded conditions. The theoretical model of kinetic ligation suggests that intermolecular attraction due to molecular crowding is involved in the optimal balance of ligation speed and available ligase. Our findings indicate that crowding effects enhance the synthesis of long DNA with large amounts of genetic information retained.